Description

A.B.T.™ 2X One-Step qRT-PCR MasterMix (with SYBR)

This product contains 2X One-Step qRT-PCR MasterMix. SYBR Green is also included.

Description: A.B.T.™ 2X One-Step qRT-PCR MasterMix (with SYBR) is an optimized solution for one-step qRT-PCR applications, incorporating SYBR-Green dye. The MasterMix is ready for use. A genetically modified M-MLV RTase, antibody-mediated hot-start Taq DNA Polymerase, MgCl2, dNTPs, stabilizer, enhance and SYBR-Green dye are the components of the MasterMix. Reaction buffer in the kit allows for RT reaction temperatures up to 50°C. This situation can increase the efficiency of the reaction by melting secondary RNA structures and reducing non-specific priming. A.B.T.™ 2X One-Step qRT-PCR MasterMix (with SYBR) presents a one-step and practical assay for the detection of gene expression directly from RNA. The MasterMix is suitable for one step quantitative real-time RT-PCR, gene knockdown verification, gene expression profiling and array validation applications.

   Cat#             Size

• Q05-02-01     1 ml
• Q05-02-05     5 ml