A.B.T.™ Custom Predesigned miRNA Plate Array
This product contains 96-well miR-cDNA Synthesis Plate and miR-qPCR MasterMix Plate.
The A.B.T.™ Custom Predesigned miRNA Plate Array are ready-to-use 96-well plates containing miRNAs according to your particular requirements. Both forward and reverse PCR amplification primers are miRNA-specific and are optimized with stem-loop technology, enabling extremely sensitive and specific miRNA quantification.
Each 96-well plate contains up to 96 pairs of PCR primers (forward: miRNA-specific stem-loop primer; reverse: universal primer). Each miRNA-specific primer used in the qPCR arrays has been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted miRNA. The A.B.T.™ Custom Predesigned miRNA Plate Array have been optimized to produce high sensitivity, efficiency, and specificity.
The A.B.T.™ Custom Predesigned miRNA Plate Array uses real-time PCR technology to quantitatively measure miRNAs. The experimental procedure includes two major steps;
1. miRNA cDNA Synthesis: miR-cDNA Synthesis Plate containing predesigned miRNA specific stem-loop primer is used to reverse transcribe the miRNA.
2. qPCR Detection: miR-qPCR MasterMix Plate containing miRqGreen MasterMix specifically detects the reverse transcribed miRNA (the miRNA-specific forward primer is used with the universal reverse PCR primer).
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- Q09-01-96 96 Rxn